Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.5.3. GC-FID Analysis

AA Aisyah Nur Hanis Azhar
NA Nurul Aini Amran
SY Suzana Yusup
MY Mohd Hizami Mohd Yusoff
request Request a Protocol
ask Ask a question
Favorite

A method used to minimize the errors when an accurate weight or volume of samples is required to determine the amount of 2AP present in the extract [11,12,13], which in this study is by using 2,4,6-trimethylpyridine (TMP) in the quantitative analysis of 2AP by GC-FID. A stock solution of 250 ppm TMP is prepared by adding 10 μL of TMP to 40 mL of acetone. This stock solution will be diluted to 50 ppm before adding it to each 0.1 mL of the extracts, and then 3 μL of the samples will be injected into GC-FID. The following formula can calculate the amount of 2AP present in the sample:

Copyright and License information:  ©2022 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A