4.6. Quantification of Adiponectin and Resistin Protein Concentrations in 3T3-L1 and pmAd Cell Culture Supernatants

The murine adiponectin, resistin and MCP1/CCL2 protein concentrations were measured in duplicates by ELISA techniques (murine adiponectin and resistin DuoSet^® ELISA development systems from R&D Systems, Wiesbaden, Germany; murine MCP1/CCL2 ELISA Kit from BioLegend, San Diego, CA, USA) and are expressed as means ± standard error of the mean (SEM). The detection ranges of the applied ELISA kits were 31.2–2000 pg/mL (for adiponectin), 15.6–1000 pg/mL (for murine resistin) and 62.5–4000 pg/mL (for murine MCP-1). The coefficients of variation (CV) of values within duplicate measurements were less than 20%. For 3T3-L1, as well as pmAd cell culture supernatants, the adiponectin and resistin levels were normalized to total cellular protein content in each well.