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2.6. Cell viability using LIVE-DEAD assay

FF Farzaneh Fouladgar
FM Forough Ghasem Zadeh Moslabeh
YK Yashesh Varun Kasani
NR Nick Rogozinski
MT Marc Torres
ME Melanie Ecker
HY Huaxiao Yang
YY Yong Yang
NH Neda Habibi
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The LIVE-DEAD assay was performed utilizing the LIVE/DEAD Viability/Cytotoxicity Kit, following the protocol provided by the manufacturer. In brief, the cells were incubated with a mixture of calcein-AM and ethidium homodimer-1 for 30 min at room temperature. Subsequently, the cells were imaged using a fluorescence microscope. The green fluorescence emitted by calcein-AM indicated live cells, while the red fluorescence emitted by ethidium homodimer-1 indicated dead cells. ImageJ software was employed to quantify the percentage of live and dead cells. All experiments were carried out in triplicate to ensure reliability and consistency of results [25].

Copyright and License information:  ©2023 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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