Experimental model and study participant details

The APOL1-KO, APOL3-KO and APOL1Δ podocyte cell lines were derived from immortalized wild-type human podocytes¹³¹ through CRISPR-Cas9-mediated editing of the APOL1 or APOL3 genes, as described in Uzureau et al.¹¹ and repeated below. Cells were grown to confluence at 33°C, at which point they were trypsinized and reseeded in fresh flasks at a dilution of between 1:3 and 1:5. Before thermoswitching to 37°C, cells were grown to 70 to 80% confluence. At both temperatures, cells were fed with fresh medium 3 times per week. Podocytes were used after 7 to 14 days of differentiation.