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Nucleosome reconstitution
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Recombinant nucleosomes with Widom 601 147-bp DNA and Xenopus laevis histones were reconstituted as described previously (Dyer et al. 2004). Briefly, individual histones were purified under denaturing conditions and refolded into histone octamers in the presence of 2 M NaCl. DNA was added and the salt was dialyzed out to yield folded nucleosome core particles. Reconstituted nucleosomes were stored at 4oC.
Copyright and License information: ©2023
This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
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