The “limma” package was employed to identify differentially expressed genes (DEGs) with these criteria of p < 0.05 and |log2FC| > 1. A univariate Cox analysis was performed to identify prognostic genes, which were used in subsequent analyses. Mutation characteristics of prognostic PCD genes were described using the “mafTools” package. The copy number variation (CNV) characteristics of these genes were visualized through the GISTIC algorithm and the “RCircos” package.
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