2.6. Functional annotation of the DEPs

JW Junqi Wang
ML Meijie Liu
CM Chengzhi Mao
SL Sizhu Li
JZ Jiabao Zhou
YF Yaqin Fan
LG Lizhong Guo
HY Hao Yu
XY Xiuqing Yang
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The DEPs were annotated and functionally enriched using Uniprot and Pfam. We also performed GO and KEGG analyses to annotate the DEPs. TBtools was used for the enrichment analyses.

The GO tool (http://geneontology.org; accessed on May 2023) was used and different terms were enriched. All terms were divided into three classes cellular components (CC), molecular functions (MF), and biological processes (BP).

The DEPs mapping to the KEGG pathways were retrieved by blasting against the KEGG database (https://www.genome.jp/kegg/pathway.html; accessed on May 2023). The pathways were attributed to several classes, such as Metabolism (A09100), Brite hierarchies (A09180), Genetic information processing (A09120), and Cellular processes (A09140).

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