After 40 weeks of HFD, we measured portal pressure in sham-operated and shunted mice (n = 4 group CD; n = 10 group HFD; and n = 10 group HFD/shunt). After a midline laparotomy, the ileocolic vein was cannulated using 27G BD Valu-Set (BD, Belliver Industrial Estate, Plymouth, UK) and connected to a calibrated digital pressure analyser (Micro Pressure Transducer with Amplifier; Radnoti Monrovia, CA, US). Portal pressure was continuously monitored, and the average over 30 seconds was recorded. Upon sacrifice of the animal, the spleen and the liver were removed, weighed, and liver/spleen weight ratio was calculated.
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