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scRNA Sequencing.

MW Matthew J. Wither
WW William L. White
SP Sriram Pendyala
PL Paul J. Leanza
DF Douglas M. Fowler
HK Hao Yuan Kueh
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Cells were stimulated for 30 h and then stained with one of six hashtag (HTO) antibodies for multiplexing. Single-cell library preparation was done with the 10× Genomics platform (95). Paired-end sequencing was performed on a NextSeq2000 (Illumina). Analysis was done with Monocle3 (v 1.2.9) (96), and gene–gene correlations were quantified using LASSO regression (71) in R.

Copyright and License information:  ©2023 the Author(s). Published by PNAS.
This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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