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Rechallenge protocol

RC Rubí M.-H. Velasco Cárdenas
SB Simon M. Brandl
AM Ana Valeria Meléndez
AS Alexandra Emilia Schlaak
AB Annabelle Buschky
TP Timo Peters
FB Fabian Beier
BS Bryan Serrels
ST Sanaz Taromi
KR Katrin Raute
SH Simon Hauri
MG Matthias Gstaiger
SL Silke Lassmann
JH Johannes B. Huppa
MB Melanie Boerries
GA Geoffroy Andrieux
BB Bertram Bengsch
WS Wolfgang W. Schamel
SM Susana Minguet
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Nalm6 cells were irradiated with 40 Gy and incubated for 24 h at 37 °C (day 0). Then, CAR T cells (5–7 d after lentiviral transduction) were added in a 1:1 ratio (day 1). After 48 h, new Nalm6 cells were irradiated with 40 Gy and incubated for 24 h at 37 °C (day 3). Then, the same CAR T cells were counted and adjusted in a 1:1 ratio with the newly irradiated Nalm6 cells (day 4). The same process was repeated until CAR T cells had three contacts with the Nalm6 cells, always having 3 d between each of them. Experiments were performed 24 h after the last contact.

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