2.5. Mutation analysis

YL Ying Li
YF Yu Fu
YQ Yichuan Qiu
QL Qian Liu
MY Ming Yin
LZ Luhua Zhang
request Request a Protocol
ask Ask a question
Favorite

The sequences of ramR, oqxR, and rpsJ in the tested strains were aligned with the reference sequence of tigecycline-susceptible isolate K. pneumoniae MGH 78578 (GenBank accession no. CP000647). The tet(A) variant was identified by comparing to the original tet(A) in plasmid pUUH239.2 (Accession no. NC_016966).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A