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On the day of sacrifice, blood was collected by retroorbital bleeding and glucose was measured using a handheld Abbott® Freestyle glucometer and test strips. Plasma was collected and ELISA kits were used to measure insulin (ALPCO Diagnostics, Salem, NH, USA), leptin, IGF-1, ADIPOQ (R&D Systems, Minneapolis, MN, USA), and FGF21 (Millipore Corp., Billerica, MA, USA).
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