Plant material

Six genotypes of wheat (Triticum aestivum L.): Zemunska rosa, Apche, Titan SBE I, Titan SBE II, W1 and Waxy1, with different amylose content (0–36.5 %), genetic background and geographical origin were used in this study. All investigated genotypes were grown in the experimental field of the Maize Research Institute Zemun Polje, Belgrade, Serbia (44˚52´N, 20˚19´E, 81 m ASL), during the 2021/2022 growing season. Standard cultivation practices were followed to provide adequate nutrients to the plants and to keep the plots free from diseases and weeds. Whole wheat flour was obtained by milling in a laboratory mill (Perten MILL 120 CE; Perten Instruments, Hägersten, Sweden) for fine sample preparation (mesh size 0.5 mm).