Cell lines and effector cells

Cell lines used were human skeletal muscle myoblasts (HSMM obtained from Lonza [6F4528]) (HLA-A*0101and Titin positive, MAGE-A3 negative), colorectal adenocarcinoma cells (Colo205 obtained from ATCC [CCL-222]) (HLA-A*0101 positive, MAGE-A3 and Titin negative), multiple myeloma cells (EJM obtained from DSMZ [ACC560]) (HLA-A*0101 and MAGE-A3 positive, Titin negative). Expression of MAGE-A3 and Titin was determined by RT-qPCR analysis (data not shown), and HLA class I status was determined by PCR-SSOP typing (ProImmune). Cells were cultured in suitable conditions recommended by the supplier. T2 cells were obtained from ATCC and transduced in-house with HLA-A*0101 by lentiviral transduction. HLA-A*0101 levels were confirmed by FACS staining. Healthy donor PBMC were used as effector cells which were obtained from Tissue Solutions (AccuCell, Precision Bioservices) or isolated from buffy coats obtained from the National Blood Service.