Lentiviral transfection of hDPCs and hiPSC-DPCs

AR Andrei Riabinin
EK Ekaterina Kalabusheva
AK Anastasia Khrustaleva
MA Mikhail Akulinin
AT Alexander Tyakht
EO Egor Osidak
EC Elina Chermnykh
AV Andrey Vasiliev
EV Ekaterina Vorotelyak
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HDPCs and hiPSC-DPCs were incubated with red fluorescent protein (RFP)-tagged (TagRFP) viral particles (Eurogen, Moscow, Russia) in tenfold excess relative to the concentration of cells in AmnioMAX-II medium (Gibco) supplemented with 10 µg/ml polybrene (Sigma).

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