Cells (2.5 × 104) were inoculated with rMV-EGFP-SLAMblind at a MOI of 1 and cultured at 37 °C. At 2, 3 and 7 dpi, cell viability was determined using the WST-1 cell proliferation kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s protocol. The viability of infected cells was expressed as a percentage by comparison of the noninfected cells.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.