The supernatant of stimulated BMDCs was collected, and mouse TNF-α (MULTI SCIENCES, EK282), IL-1β (MULTI SCIENCES, EK201BHS), IL-6 (Bio-Techne ValukineTM, VAL604G) and IL-12p70 (MULTI SCIENCES, EK212HS) ELISA kits quantified the cytokines released into the supernatant with a standard protocol.
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