The FASTA format trimmed files of ITS2 and COI after aligning were converted to Nexus alignment (.nex) and Phylip alignment (.phy) file formats by Geneious Prime 2022.1.1 software. The best schemes of models of evolution for ITS2 and COI sequences were obtained using PartitionFinderV1.1.1_Windows software as shown in Table 3. Phylogenetic trees of ITS2 and COI were generated separately using obtained best models of evolution in MrBayes- V 3.2.5_ Bayesian inference of Phylogeny software.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.