2.5. Total Protein Quantification

Loredana Stanca; Ovidiu Ionut Geicu; Andreea Iren Serban; Anca Dinischiotu

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

2.5. Total Protein Quantification

LS Loredana Stanca

OG Ovidiu Ionut Geicu

AS Andreea Iren Serban

AD Anca Dinischiotu

This method is extracted from research article: Materials (Basel), Jul 2023

Interplay of Oxidative Stress, Inflammation, and Autophagy in RAW 264.7 Murine Macrophage Cell Line Challenged with Si/SiO2 Quantum Dots

DOI: 10.3390/ma16145083

Ask a question

Favorite

For protein concentration determination of the cell lysates, the Bradford protein assay kit (Bio-Rad) was used according to the manufacturer’s instructions. Optical density was measured at 595 nm using a TECAN GENios multireader (TECAN Group Ltd., Männedorf, Switzerland) equipped with Magellan v7.0 software, and a standard curve was constructed using bovine serum albumin (BSA).

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol