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2.5.2. Cryo-Transmission Electron Microscopy (Cryo-TEM) Images

MB Marina Beraza-Millor
JR Julen Rodríguez-Castejón
JM Jonatan Miranda
AP Ana del Pozo-Rodríguez
AR Alicia Rodríguez-Gascón
MS María Ángeles Solinís
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Cryo-transmission electron microscopy (Cryo-TEM) was performed using a TECNAI G2 20 TWIN (FEI), operating at an accelerating voltage of 200 KeV in a bright-field image mode and low-dose image mode. Three µL of the sample was placed on glow-discharged 300 mesh Quantifoil TEM grids and used for plunge freezing into liquid ethane on an FEI Vitrobot Mark IV (Eindhoven, The Netherlands). The frozen grids were transferred to a 626 DH Single Tilt Cryo-Holder (Gatan, France), where the temperature was below −170 °C, and then to TEM at liquid nitrogen temperature.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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