2.4.2. Assessment MICs of Sorghum Extract against C. jejuni

Sorghum extracts of minimum inhibitory concentrations against C. jejuni EMCC 1835 were evaluated using agar disk diffusion assay [22,25] with the following descending concentrations of white sorghum extract: 100, 50, 25, 12.5, 6.25, 3.12 µL. C. jejuni spread on MHM plates of grown Mueller Hinton Medium (MHM) plates and adjusted to a density of 10⁶ CFU/mL [26] and the plates were kept at a temperature of 4 °C for 30 min and then incubated at 42 °C/24 h. A clear and sharp inhibition zone was noted in mm, taking in consideration the anti-C. jejuni potential of the different sorghum extracts.