2.4.5. Estimation of Steroids

SA Syed Kashif Ali
HM Hafiz A. Makeen
GK Gulrana Khuwaja
HA Hassan A. Alhazmi
MS Mukul Sharma
AK Afraim Koty
IM Islam Mazahirul
HP Humaira Parveen
AM Asaduddin Mohammed
SM Sayeed Mukhtar
MA Mohammad Firoz Alam
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Steroids were detected as per a previously described method [25]. Briefly, one gram of extract was dissolved in a few drops of acetic acid and acetic aldehyde, warmed and cooled under running water, and a drop of sulfuric acid was applied along the test tube’s edges. Steroids are present when a reddish brown ring forms at the contact.

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