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2.3.1. Extraction and Estimation of Pigments, Total Soluble Sugars, and Malondialdehyde

BA Basmah M. Alharbi
AA Awatif M. Abdulmajeed
AJ Alae A. Jabbour
AH Ahmed M. Hashim
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The pigment content in terms of chlorophyll a, chlorophyll b, and carotenoids were extracted and calculated according to the method of Metzner and colleagues [37]. Total soluble sugars were extracted following the method of Homme et al. [38] and measured according to Loewus [39]. Following the method of Heath and Packer [40], the quantity of lipid peroxidation was assessed by counting the MDA amount generated by the thiobarbituric acid reaction.

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