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4.11. Animal Models

DC Dylan Chapeau
SK Sofia Koustoulidou
MH Maryana Handula
SB Savanne Beekman
CR Corrina de Ridder
DS Debra Stuurman
EB Erik de Blois
YB Yulia Buchatskaya
KS Karlijn van der Schilden
MJ Marion de Jong
MK Mark W. Konijnenberg
YS Yann Seimbille
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Six-week-old male Balb/c nu/nu-specific and opportunistic pathogen-tree (SOPF) mice (Janvier Labs, Le Genest-Saint-Isle, France) were housed in individually ventilated cages, with four mice per cage. Upon arrival, the mice were acclimated for 1 week with access to food and water ad libitum. Mice were subcutaneously inoculated on the right shoulder with NCI-H69 cells (5 × 106 cells suspended in 100 μL of 1/3 Matrigel (Corning Inc.; Corning, NY, USA) and 2/3 Hank’s balanced salt solution (Gibco; Paisley, UK). NCI-H69 xenografts were allowed to grow for 3 weeks. Tumor sizes were 391 ± 173 mm3 at the start of the studies.

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