2.8. Caspase-1 Inflammasome Assay

Caspase-1 activity was determined using a Caspase-Glo^®1 inflammasome assay kit (Promega Corp., Madison, WI, USA). Briefly, 5 × 10⁴ cells were treated with an IC₅₀ dose of salamandrin-I for 48 h. After incubation, 100 μL of the Caspase-Glo 1 reagent, with and without the caspase-1 inhibitor YVAD-CHO, was added to the plate and mixed using a plate shaker at 300 rpm for 30 s. The samples were incubated at room temperature, and luminescence was measured after 60 min using a Multimode Plate Reader (PerkinElmer, Waltham, MA, USA).