4.6. Expression Analysis of Chlorophyll Biosynthesis and Degradation Gene in Light Deficiency Stress and MsSGR in Different Tissues

The expression patterns of chlorophyll biosynthesis and degradation genes and MsSGR in different tissues were assayed using qRT-PCR and the primers were designed using Primer 5.0 (Table S1). The common isoform sequences were used to design primers for qRT-PCR (Figure S10). The qRT-PCR experiment was conducted using BCG qPCR Master Mix (Beijing Baikaiji Biotechnology, Beijing, China) on a LightCycler^® 480 II (Roche Applied Science, Penzberg, Germany). The qRT-PCR program was as follows: 95 °C for 2 min, 40 cycles of 95 °C for 15 s, and 60 °C for 40 s. EF1-α was selected as the reference gene to normalize the gene expression, and the relative gene expression was determined using the 2^−ΔΔCt method [60]. All the qRT-PCR analysis experiments were performed in triplicate. The bar charts of gene expression were generated using Origin 2018 (OriginLab Corporation, Northampton, MA, USA), and SPSS 24.0 (SPSS Inc., Chicago, IL, USA) was used to analyze statistical significance.