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Reverse transcription-polymerase chain reaction (RT-PCR)
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The NPCs were seeded in six-well plates based on a cell density of 2×105 wells. Following successive treatment of the corresponding groups with different concentrations of LPS, the cells were lysed with Trizol, and the RNA was extracted. RT-PCR was used to assay Col-2, Aggrecan, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression levels, respectively, with GAPDH as an internal reference.
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