2.5. RNA Isolation and cDNA Synthesis

After photobleaching started appearing in true leaves of TRV2-GrCla-infiltrated plants, total RNA was isolated from true leaves of non-infiltrated control, TRV:00-, TRV2-WRKY25-, and TRV2-WRKY33-infiltrated plants. RNA extraction protocol recommended by the supplier (Invitrogen, Carlsbad, CA, USA) of RNA extraction kit TRIzol Plant RNA isolation reagent (Invitrogen) was used for the isolation of total RNA from cotton leaves. RNA samples were quantified followed by treatment with DNase to remove any traces of plant genomic DNA. The cDNA was prepared from RNA using RevertAid First-strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat No. K1621).