2.6. Proliferation Assay (xCELLigence)

NG Natalia Garcia-Becerra
MA Marco Ulises Aguila-Estrada
LP Luis Arturo Palafox-Mariscal
GH Georgina Hernandez-Flores
AA Adriana Aguilar-Lemarroy
LJ Luis Felipe Jave-Suarez
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Cells (5000) were seeded in triplicate in an xCELLigence Real-Time Cell Analysis (RTCA) 96-well electronic microplate (E-Plate 96) (Cat. No. 300601010, Agilent Technologies, Inc., Santa Clara, CA, USA) and incubated for 48 h with impedance detection every hour with the xCELLigence RTCA SP station (Agilent Technologies, Inc., Santa Clara, CA, USA). Proliferation was measured as cell index change over time.

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