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MTT assay

RS Ram Krishna Sahu
ST Simran Tandon
SS Shalini Singh
BD Bhudev Chandra Das
SH Suresh T Hedau
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The efficacy of resveratrol was assessed on MCF-10A breast normal cell line, MCF-7, T-47D & MDA-MB-231 breast cancer cell lines by MTT assay (Sigma, USA) and stained with MTT dye (Sigma, USA). A 100mM working stock was prepared and treated with a different concentrations in µM. MTT assay was performed in a 96-well plate; cells were counted using a hemocytometer with an equal volume of trypan blue dye. 5,000 cells seeded in each well of 96 well plates and kept in an incubator overnight, next day treated with different concentrations (10-50µM) of resveratrol and incubated for 24 hours. then 20µL MTT dye (5mg/mL) was added to each well and incubated for 4 hours after that lysis solution was added, mixed well and incubated for 1 hours again finally read at 570nm wavelength by a microplate reader. From absorbance values calculated the percentage of viable cells [24].

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