2.6. Oxygen radical absorbance capacity (ORAC)

ORAC assay was determined following the method proposed by Huang et al. [22] with some modifications. The reaction was performed in a 96-well microplate reader at a final volume of 210 μL. The reaction mixture contained 150 μL of fluorescein (40 nM) and 30 μL of extract or standard (0.5 to 15 μM Trolox in PBS at pH 7.4). Before the addition of 2,2′azobis (2methylpropionamidine) dyhydrochloride-AAPH (30 μL of 19 mM AAPH in PBS at pH 7.4), the mixture was tempered at 37 °C for 15 min in the microplate reader.