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Extraction and quantification of free AAs
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The free AAs in tea plant tissues were extracted from 120 mg tea tissues with 1 mL of 4% sulfosalicylic acid as described previously (Zhang et al., 2021). The supernatants were filtered through a 0.22 μm Millipore filter before analysis. A mobile phase containing lithium citrate for AA derivatization and UV–Vis detection at 570 and 440 nm were used in the Hitachi High-Speed Amino Acid Analyzer system (L-8900, Hitachi) (Zhang et al., 2021). The peak areas of AAs were quantified in comparison with the AA standards.
Copyright and License information: ©2024 The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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