Experimental model and study participant details

Androgen-positive LNCaP, VCaP, C4-2B and 22RV1, and androgen-negative PC3 and DU145 cells were amplified and frozen upon receipt. LNCaP, 22RV1, DU145 and PC3 cell lines were cultured in RPMI1640 culture medium supplemented with 10% fetal bovine serum and 1% of Penicillin/Streptomycin 5000 U/mL. VCaP cells were cultured in DMEM culture medium supplemented with 10% fetal bovine serum and 1% of Penicillin/Streptomycin 5000 U/mL. C4-2B cells were cultured in DMEM/F12 (4:1, v/v) culture medium supplemented with 10% fetal bovine serum, 0.1 μg/mL insulin, 275 ng/mL triiodothyronine, 88.6 ng/mL apo-transferrin, 4.9 ng/mL d-Biotin, 252 ng/mL adenine and 1% of Penicillin/Streptomycin 5000 U/mL. Cells were cultured in a humidified incubator at 37°C with 5% CO2 and were routinely tested negative to Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit. All commercially obtained cells were authenticated by the vendors. All compounds were dissolved in DMSO.