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OCT imaging

TL Tamar Azrad Leibovitch
NF Nairouz Farah
AM Amos Markus
YM Yossi Mandel
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GCaMP6f+\−RCS-\- rats were anesthetized with i.m 1 mg/kg ketamine (Ketamidor, Richter Pharma) and 0.5 mg/kg xylazine (Sedaxylan, Eurovet Animal Health) after which the pupils were dilated. Optical coherence tomography images (OCT, Spectralis, Heidelberg) were acquired and served to measure the thickness of the various retinal layers (ONL, ONL + OPL + debris, INL, and whole retina), as a part of a long-term follow-up (4, 8, and 12 weeks). In addition, confocal scanning laser ophthalmoscopy (cSLO) fluorescence imaging was performed to visualize the fluorescence induced by GCaMP6f gene expression in the RGCs.

Copyright and License information:  ©2024 Azrad Leibovitch, Farah, Markus and Mandel.
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