After the dsRNA injection described above, the insects were individualized and weighed before and after the blood meal at different time points. Eggs laid were collected on the same days the animals were weighed for analysis of oviposition, and, approximately 15 days later, the hatched nymphs were counted. Finally, the longevity of females was measured by observing the day each insect died.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.