2.5.2. Cell proliferation

The alloys were extracted using a complete culture medium at a concentration of 0.1 g/mL for 24 h, while the Mg–Ca/nHAC group was extracted at a ratio of 0.1 g/mL (Mg–Ca) and 60 mg/mL (nHAC). After filtering and sterilizing, the experimental groups were divided into three categories: the Control group, the groups with an extraction solution to culture medium ratio of 1:2, and the groups with an extraction solution to culture medium ratio of 1:1. The RAW264.7 cells were harvested during the logarithmic growth phase and then plated at a density of 1 × 10⁴ cells/well in a 96-well plate. The cells were cultured at 37 °C in 5 % CO₂ for 24, 48, and 72 h. Following this, the culture medium was removed, and each well was washed three times with 100 μL PBS. Subsequently, each well was incubated with 100 μL of CCK-8 solution (Solarbio, China) at 37 °C for 2 h. The absorbance was measured at 450 nm.