Karyotype analysis and result interpretation

Suitable karyotypes were scanned using a Zeiss automated scanning analyzer (sensitivity coefficient set at 7.0, scanning the entire slide), and karyotype analysis was performed using the iKaryoS software. Samples that successfully completed karyotype analysis were recorded as “+”, samples with no analyzable karyotype were recorded as “-”, and samples with karyotypes present but with substandard banding or insufficient numbers for analysis were recorded as “±”.