2.8.3. Assessment of malondialdehyde

Malondialdehyde (MDA; a product of polyunsaturated fatty acids peroxidation) was determined as described by Du and Bramlage (Du and Bramlage, 1992) with slight modification. Briefly, MDA was extracted from approximately 500 mg of homogenized fresh tissue using 20% trichloroacetic acid (TCA) containing 0.01% butyl hydroxyl toluene (BHT), incubated at 95°C, then centrifuged at 10,000 ×g for 10 min. MDA content was colorimetrically determined in the collected supernatants by measuring the absorbance at 532 and 600 (A₅₃₂ and A₆₀₀ nm) using UV-160A spectrophotometer (Shimadzu, Japan), then expressed as nmol g⁻¹ FW.