Quantitative real-time polymerase chain reaction (qRT‒PCR)

GL Guoxin Liang
CG Chang Guo
HT Hongyue Tang
MZ Mingming Zhang
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Total RNA was extracted from H9c2 cells via TRIzol reagent and quantified by spectrophotometry. For miRNA detection, 1 μg of total RNA was reverse transcribed in the presence of a stem ring and amplified using a Bulge-Loop TM miRNA qRT‒PCR Starter Kit (C10211–2, RiboBio Biotechnology, Guangzhou, China). The most stable expression, U6, was the endogenous control, and the test was repeated three times. The Ct value was calculated and analyzed by the 2−△△Ct method, and the relative expression level of miR-30a-5p was determined.

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