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Subsequently, with a time gap in the order of minutes, the second imaging was conducted using a prototype SSOCT system (PlexElite 9000, Zeiss Meditec, Dublin, California, USA) with a central wavelength scanning laser of 1,050 nm, system operation speed of 100,000 to 200,000 A-scan/s, and axial and lateral resolutions in tissue of 6.3 µm and 20 µm respectivelyEach SSOCT data volume consists of 300 A-scans and 300 B-scans covering a 3 mm x 3 mm x 3 mm volume.
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