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In-vivo analysis of peptide efficacy

KB Komal Beeton
DM Dipanwita Mitra
AA Adesanya A. Akinleye
JH John A. Howell
CY Christian S. Yu
GI Gene L. Bidwell, III
RT Ritesh Tandon
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Mice (3 males, 3 females per treatment group) were anesthetized with isoflurane and subcutaneously injected with 1000 nmol/Kg of the P10, ELP, ELP-P10, or vehicle-treated 1 h prior to infection. One-hour post-treatment, mice were infected intraperitoneally with 1 × 106 PFU of MCMV K181. Animals were treated with the same dose of peptides every 24 h, and their weight was monitored. Three days post-infection, animals were euthanized, and the liver, kidneys, spleen, heart were harvested and stored at − 80 °C. Virus titers in harvested organs were determined by plaque assay.

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