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MEFs were used to culture the K181 strain of MCMV. To prepare the virus stock, the MCMV was mixed with 3× autoclaved milk, which was derived from Carnation (Nestle) instant nonfat dry milk powder. The mixture was sonicated three times for 10 s each, with a 30-s gap in between, and then stored at − 80 °C. Additionally, 10% milk was prepared in nanopure water, with a pH of 7.0, and autoclaved three times.
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