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After trypsin digestion, the cells were collected from cultures incubated with 0 mM, 2.5 mM, 5 mM, 7.5 mM or 10 mM Nacr for 4 h, 8 h or 12 h. Then, 10 µl of 0.4% Trypan blue stain (Thermo Fisher Scientific, T10282) was added to the resuspended cells (10 µl), and the mixture was thoroughly mixed before adding 10 µl of the cell suspension to the cell counting plate. The percentage of dead cells was determined using a cell counter (Life Technologies Countess II, USA).
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