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Hi-C library preparation and sequencing

LY Luo Yingning
WS Wei Shuhua
DW Dai Wenting
MM Miao Miao
WY Wang Ying
ZR Zhang Rong
BL Ban Liping
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The Hi-C sequencing library was prepared with 800 mixed-sex adult thrips. Samples were cross-linked with a 2% formaldehyde isolation buffer and then treated with DpnII (New England Biolabs, Beijing, CN) to digest nuclei. Biotinylated nucleotides were used to repair tails, and the ligated DNA was split into fragments of 300–700 bp in length. The resulting Hi-C library was sequenced in Illumina Novoseq 6000 for 150 bp paired-end reads. After applying the same filter criteria for short reads, a total of 31.78 Gb (~93 × coverage) of clean data was generated to assist the chromosome-level assembly (Table 2).

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