Evaluation of growth factor levels (ELISA assay)

JN Jintao Ni
DY Danyan Ye
WZ Weiping Zeng
SM Siyi Ma
ZW Zhixia Wang
YK Yuping Kuang
LY Lujun Yang
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The CM of HuMSCs at 100% confluence, HuMSCs-CM (Day 3), and GS-HuMSCs-CM (Day 3) were collected, and the levels of VEGF, IGF-1, KGF, and HGF in CM were quantified by ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions. All data were compiled from a minimum of three replicate experiments, with each performed in duplicate, and growth factor levels were calculated from four-parameter logistic curves. Finally, the growth factor concentration was adjusted to be ng (or pg) /ml/million cells.

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