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Biological evaluation

YL Yanfeng Liu
LD Liangying Deng
FD Feng Ding
QW Qiang Wang
SZ Shuran Zhang
NM Nana Mi
WZ Wenhui Zhang
BZ Bailin Zeng
HT Huangjin Tong
LW Lixing Wu
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The anti-CETP activity of the molecules was measured using a standard fluorescent CE-transfer assay (CETP Inhibitor screening kit [ab283403]; Abcam, Cambridge, UK). Briefly, the compounds were purchased from Bidepharm (Shanghai, China), fully dissolved in dimethyl sulfoxide (Sigma–Aldrich, St. Louis, MO, USA), and stored in a nitrogen-filled cabinet. A solution without recombinant CETP (rCETP) served as the background. The positive controls contained rCETP but no test compounds. In the assay buffer, the donor (4 mL), acceptor (4 mL), and test drugs (1 mL) were mixed with 30 ng rCETP (200 mL). The fluorescence intensity was measured in kinetic mode using a fluorimeter (Agilent BioTek Synergy H1 Multimode Reader) at Ex/Em = 480/511 nm. The inhibition ratio was computed after a 30-min incubation at 37 °C. The results of the CETP inhibition assay are presented in Additional file 1: Table S2. Furthermore, graphical representations of the data were generated using GraphPad software.

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