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Quantification of culturable mesophilic aerobic bacteria
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The saline swab was used to quantify the culturable mesophilic aerobic bacteria. To do this, the 0.85% saline solution tube containing the swab (designated “0”) was vigorously mixed using a homogenizer vortex and diluted to 10− 2 in 0.85% saline. A volume of 100 µL of “0” and each dilution was then spread on the surface of standard agar plates for counting, in triplicate. The plates were then incubated at 37 °C for 48 h, after which the number of colony-forming units (CFU)/mL was determined.
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