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Behavioral tests

ZX Zhehao Xu
YW Yue Wang
CJ Changcheng Jiang
ZW Zhengwei Wang
YC YongFeng Cheng
MF Manli Fan
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Scratching behaviors experiments were performed as previously described. 24 The mice were placed individually in separate plastic chambers (10 cm × 10 cm × 13 cm) and allowed to acclimatize for 30 min. In the mouse model of acute itch, the scratching behavior was video recorded for 30 min after the intradermal injection of BMS-1 or BMS-202 in the nape of the neck. Intradermal injection of PD-L1 was administered in an experimental setup to observe PD-L1 effect on imiquimod-induced chronic psoriasis itch. Behavioral videos were recorded within 5 h post intradermal injection of PD-L1 on day 6 following 5 days of imiquimod treatment. The total number of scratches was counted blindly.

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