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RT-qPCR analysis

CW Chaojie Wu
DC Danling Cai
JL Jun Li
ZL Zengxiang Lin
WW Wei Wei
WS Wei Shan
JC Jianye Chen
WL Wangjin Lu
XS Xinguo Su
JK Jianfei Kuang
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Extraction of total RNA was carried out by the procedure previously reported [40]. RT-qPCR was carried out using the GoTaq qPCR master mix kit (Promega, Madison, WI, USA) in a CFX96 Real-Time PCR Apparatus (Bio-Rad, Hercules, CA, USA) following the manufacturer’s protocol. MaRPS4serves as an endogenous reference gene [64]. The primer sequences used are available in Table S1 (see online supplementary material).

Copyright and License information:  ©2024 The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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