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HCEC-21T or FECD-54F cells lines were seeded at a density of 300,000 cells per well in 12 well plates pre-coated with undiluted fibronectin coating mix overnight. A P-200 pipette tip was used to generate a linear scratch. Media was removed and replaced with fresh cell culture media. Images were captured every 2 h at 10 × magnification for 24 h with the Incucyte S3 Live-Cell Analysis system (Sartorius). Scratch assay images were analyzed using ImageJ or T-Scratch.
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